Reporter

Part:BBa_K088006:Design

Designed by: Hai Chen   Group: iGEM08_CPU-NanJing   (2008-10-13)


lacI+pL+cI+GFP+Pr+mRFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1164
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 3701
    Illegal AgeI site found at 3813
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2850


Design Notes

In the absent of lactose or IPTG,mRFP is expressed. In the presence of lactose or IPTG,GFP is expressed, while mRFP not.


Source

BBa_R0010+BBa_J24679+BBa_C0051+BBa_E0240+BBa_I763007

References